ABSTRACT
Objective: To analyze the chemical constituent cluster of decoction of Sanguisorba Radix systemically by HPLC-IT-TOF/MS. Methods: The samples were scanned by broad spectrum of DAD detector and ionized in negative and positive environment of electron spray ionization. Results: A total of 82 chemical constituents (include isomers) were identified based on exact molecular mass, fragment, ultraviolet spectrum as well as the combination of the database. The chemical constituent cluster was composed of 69 phenolics, 8 triterpenes, 3 flavonoids, an organic acid, and a monoterpene glycoside, of which citric acid, brevifolin carboxylic acid, methoxybenzoic acid methyl ester-5-O-sulfate, isorhamnetin-sulfate, and methylellagic acid-sulfate, et al were firstly found in Sanguisorba Radix. Conclusion: The systemical analysis of the chemical constituent cluster of decoction of Sanguisorba Radix was able to supply a clear material basis for the further study of efficacy and pharmaceutical metabolism of Sanguisorba Radix.